昆明东大河湿地与捞鱼河湿地春秋两季浮萍根系微生物群落组成及丰度的研究

楚雄师范学院学报 ›› 2021, Vol. 36 ›› Issue (3): 51-59.

昆明东大河湿地与捞鱼河湿地春秋两季浮萍根系微生物群落组成及丰度的研究

孔令冬, 张昱, 张洪波, 赵明芳, 夏云^*

昆明学院农学与生命科学学院,云南昆明 650214

收稿日期:2021-01-02 出版日期:2021-05-20 发布日期:2021-07-23
通讯作者: *夏云（1966–）,女,博士,昆明学院农学与生命科学学院教授,主要研究方向为分子微生物学。E-mail： 3098933205@qq.com
作者简介:孔令冬（1996–）,女,硕士研究生, 主要研究方向为微生物资源利用。E-mail： kongld15012184664@163.com
基金资助:
云南省教育厅科学研究基金项目（No. 2021Y26,No. 2020Y0470）

Study on the Composition and Abundance of the Rhizosphere Microbiota of Lemna minor in the Dongda River Wetland and the Laoyu River Wetland of Kunming in Spring and Autumn

KONG Lingdong, ZHANG Yu, ZHANG Hongbo, ZHAO Mingfang, XIA Yun^*

College of Agriculture and Life Sciences, Kunming University, Kunming, Yunnan Province 650214

Received:2021-01-02 Online:2021-05-20 Published:2021-07-23

摘要/Abstract

摘要： 为了探明晋宁东大河湿地及捞鱼河湿地浮萍根际微生物群落在春、秋两季的组成、功能和丰度之间的联系,实现湿地资源利用的可持续发展, 以及生物多样性的保护并为生物灾害预防提供警示, 本研究采用荧光原位杂交技术对两个湿地的浮萍根际微生物进行了研究。通过测定春秋两季东大河湿地与捞鱼河湿地浮萍根系微生物群落的组成和相对丰度,并对不同时空浮萍根系微生物群落中目标微生物丰度进行了差异性分析。结果发现,除绿弯菌不受生境及季节变化的影响外,氨氧化细菌、反硝化细菌、硫酸盐还原细菌、产甲烷细菌、变形菌、拟杆菌的丰度均不同程度的受生境及季节变化的影响。监测浮萍根际微生物组成和丰度的变化,不仅可以作为水体监控防治的生物指标,还可用作一项有效的湿地水质常规管理中的技术支持。

关键词: 湿地资源利用, 浮萍根际微生物, 功能菌群, 荧光原位杂交

Abstract: To obtain the compositional and functional information of the rhizosphere microbiota of Lemna minor in the Dongda River Wetland and the Laoyu River Wetland of the Dianchi Lake of Kunming, so as to achieve sustainable development and utilization as well as biodiversity protection of wetland resources and provide biological disaster prediction, fluorescence in situ hybridization was used to investigate the rhizosphere microbiota of Lemna minor in these two wetlands during spring and autumn. By comparing the differences in composition and abundance of the rhizosphere microbiota of Lemna minor in these two sites, we found that except for the abundance of Chloroflexus, that of ammonia oxidizing bacteria, denitrifying bacteria, sulfate-reducing bacteria and methanogenic bacteria as well as Proteobacteria and Bacteroides varied at different degrees with changes in wetlands and seasons. The results from this study can be used not only as biological indicators of the water quality, but as an effective technology support to manage the water quality of the wetlands.

Key words: wetland resource utilization, duckweed root microorganisms, functional flora, fluorescence in situ hybridization

中图分类号:

Q949.717.3

孔令冬, 张昱, 张洪波, 赵明芳, 夏云. 昆明东大河湿地与捞鱼河湿地春秋两季浮萍根系微生物群落组成及丰度的研究[J]. 楚雄师范学院学报, 2021, 36(3): 51-59.

KONG Lingdong, ZHANG Yu, ZHANG Hongbo, ZHAO Mingfang, XIA Yun. Study on the Composition and Abundance of the Rhizosphere Microbiota of Lemna minor in the Dongda River Wetland and the Laoyu River Wetland of Kunming in Spring and Autumn[J]. Journal of Chuxiong Normal University, 2021, 36(3): 51-59.

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