滇青冈（Cyclobalanopsis glaucoides）内生菌及根际土壤微生物的初步研究

楚雄师范学院学报 ›› 2021, Vol. 36 ›› Issue (3): 67-79.

滇青冈（Cyclobalanopsis glaucoides）内生菌及根际土壤微生物的初步研究

杨海艳^1,#, 李艳双^1,#, 李蕊君², 张燕³, 罗中泽^3,*, 李天星^1,*

1.楚雄师范学院资源环境与化学学院,云南楚雄 675000;
2.楚雄师范学院语言文化学院,云南,楚雄 675000;
3.楚雄州农业科学院经济作物研究所,云南楚雄 675000

收稿日期:2019-12-03 出版日期:2021-05-20 发布日期:2021-07-23

Preliminary Study on the Endophytes and Rhizosphere Soil Microbes of Cyclobalanopsis glaucoides

Haiyan Yang^1,#, Yanshuang Li^1,#, Riujun Li², Yan Zhang³, Zhongze Luo^3,*, Tianxing Li^1,*

1. School of Resources, Environment & Chemistry, Chuxiong Normal University, Chuxiong, Yunnan Province 675000;
2. College of Language and Culture, Chuxiong Normal University, Chuxiong, Yunnan Province 675000;
3. Institute of Cash Crops, Chuxiong Academy of Agricultural Sciences, Chuxiong 675000, China

Received:2019-12-03 Online:2021-05-20 Published:2021-07-23
Contact: *Zhongze Luo （1978–）, senior agronomist, mainly engaged edible fungus cultivation; Tianxing Li（1968–）, doctor, professor. His research interests include plant physiology ecology, landscape ecology and biology education. E-mail： lyzh@cxtc. edu.cn, Tel. 15887517910
About author:#Haiyan Yang （1979–）, doctor, associate professor, mainly engaged in plant pathology, plant physiology research. E-mail： haiyanyang@cxtc. edu.cn, Tel. 18287864615; Yanshuang Li （1995–）, undergraduate, mainly engaged in biological research. E-mail： 15911630736@163.com, Tel. 15911630736

摘要/Abstract

摘要： 实验对滇青冈根、茎、叶的内生菌及根际土壤微生物进行了初步研究,结果表明：从滇青冈根、茎、叶及根际土壤中分离出细菌61株,真菌24株,共85株。根中分离出了29株,包括25株细菌,4株真菌;茎中12株,包括4株细菌,8株真菌;叶中7株,包括3株细菌,4株真菌,即根（34%）>茎（14%）>叶（8%）,其余37株都是从土壤中分离出来的。细菌在滇青冈体内分布情况为根（56%）>茎（8%）>叶（6%）,真菌则是茎中较多。从根、茎、叶中分离出的细菌多为球菌,根际土壤中分离出的则是杆菌较多,二者分离出的菌株都是G⁺占优势。分离出的真菌包括2株曲霉属真菌、2株枝孢属、1株凸脐蠕孢属、1株葡萄孢属、1株炭疽菌属、4株青霉属、2株团黑孢霉属,其他的为无孢类群,其中青霉属真菌略占优势。实验结果为滇青冈的化感作用研究提供部分数据支持。

关键词: 滇青冈, 内生菌, 根际土壤微生物

Abstract: The results showed that 61 strains of bacteria and 24 strains of fungi were isolated from the root, stem, leaf and rhizosphere soil of Cyclobalanopsis glaucoides 29 strains were isolated from roots, including 25 strains of bacteria and 4 strains of fungi; 12 strains in stems, including 4 strains of bacteria and 8 strains of fungi; 7 strains in leaves, including 3 strains of bacteria and 4 strains of fungi, namely root （34%） > stem （14%） > leaf （8%）, and the remaining 37 strains were isolated from soil. The distribution of bacteria in C. glaucoides was as follows： root （56%） > stem （8%） > leaf （6%）. Fungi were more in stem. Most of the bacteria isolated from roots, stems and leaves were cocci, while the bacteria isolated from rhizosphere soil were more than those from rhizosphere soil. The isolated fungi included 2 strains of Aspergillus, 2 strains of Cladosporium, 1 strain of Verrucaria, 1 strain of botryosporium, 1 strain of Colletotrichum, 4 strains of Penicillium and 2 strains of Colletotrichum. The others were non spore groups, among which Penicillium was slightly dominant. The results of this study can provide support for the experiment of C. glaucoides.

Key words: Key Words： Cyclobalanopsis glaucoides, endophytes, rhizosphere soil microorganisms

中图分类号:

S154.3

杨海艳, 李艳双, 李蕊君, 张燕, 罗中泽, 李天星. 滇青冈（Cyclobalanopsis glaucoides）内生菌及根际土壤微生物的初步研究[J]. 楚雄师范学院学报, 2021, 36(3): 67-79.

Haiyan Yang, Yanshuang Li, Riujun Li, Yan Zhang, Zhongze Luo, Tianxing Li. Preliminary Study on the Endophytes and Rhizosphere Soil Microbes of Cyclobalanopsis glaucoides[J]. Journal of Chuxiong Normal University, 2021, 36(3): 67-79.

参考文献

[1] Chinese Flora Editorial Committee, Chinese Academy of Sciences. Flora of China [M]. Beijing: Science Press, 2004.
[2] Yang Y, Wei K Y.Distribution and allocation model of Cyclobalanopsis glaucoides in Kunming area[J]. Guizhou Agricultural Sciences,2012,40(1):125–128.
[3] Zou Y.Seed germination of Cyclobalanopsis glaucoides[J]. Plant Physiology Journal, 2003, 39(4): 325–326.
[4] Su W H, Zhang G F, Zhang C.Biological and ecologicai studies on seed germination of Cyclobalanopsis glaucoides[J]. Seed, 2001, 5(118): 29–30.
[5] Zheng Y L, Ma H C.Temperature tolerance of Quercus variabilis ang Cyclobalanopsis glaucoides Seedlings[J]. Forestry Science & Technology, 2015, 40(5): 8–10.
[6] Cao J X, Zhang G F, Zhang L, et al. Acclimation of photosynthesis and growth different growth light conditions for seedligs of Cyclobalanopsis glaucoides[J]. Guihaia, 2008, 28(1): 126–129.
[7] Li T X, Yang H W, Zhang W X.Determination of allelopathy of overground part volatile of Cyclobalanopsis glaucoides on Triticum aesivum[J]. Seed, 2018, 37(9): 21–26.
[8] Li T X, Yang H W, Zhong W T.Determination of allelopathy effects of overground part eluviation substances of Cyclobalanopsis glaucoides on Oryza sativa [J]. World Journal Forestry, 2018, 7(2): 74–81.
[9] Li T X, Yang H W, Mao Z C.Determination of allelopathic effects of residue decomposition of Cyclobalanopsis glaucoides on Triticum aesivum[J]. International Journal of Ecology, 2018, 7(2): 45–52.
[10] Wang Z W, Ji Y L, Cheng Y G.Studies and biological significances of plant endophytes[J]. Microbiology China Tongbao, 2015, 42(2): 349–363.
[11] Cheng Z H, Xue L W, Yuan B, et al. Isolation of endophytic fungi from atractylodes lancea and their antiumor activity against HepG2 cell line[J]. Biotic Resources, 2017, 39(1): 58–61.
[12] Li LX, L D, Z L, et al. Isolation and identification of garlic endophytes and screening of their antagonistic strains[J]. Jiangsu Agricultural Sciences, 2018, 46(05): 97–101.
[13] Yi T, Miao Y X, Feng Y J.Plant-endophyte interaction: growth-promoting effect of endophtes and their biofilm formation[J]. Microbiology China Tongbao, 2008, 35(11): 1774–1780.
[14] Larran S, Simon M R, Moreno M V, et al. Endophytes from wheat as biocontrol agents against tan spot disease[J]. Biological Control, 2016, 92: 17–23.
[15] Song WW, Zhu H, Yu FY, et al. Review on plant endophytes and their control effects on plant diseases[J]. Jiangsu Agricultural Sciences, 2018, 46(6): 12–16.
[16] Li J, Zhang B L, Zhao Y, et al. Progress in endophyte improving plant salt and alkali resistance[J]. Biotechnology Bulletin, 2014, 4: 14–18.
[17] Wang M Y, Zhou D B, Jing T, et al. Endophytes isolation and broad-spectrum antagonistic bacteria screening from banana[J]. Biotechnology Bulletin, 2014, 8: 138–145.
[18] Li H Y, Liu L, Wei D Q, et al. Isolation and screening for endophytic fungi with antitumor activities form 12 Yunnan Medicinal Plants[J]. Research and development of Natural Products, 2007, 19: 765–771.
[19] Wu F, Li C R, Cui P, et al. Population analysis of mulberry rhizosphere microbes in different soil fertilities[J]. Acta Ecologica Sinica, 2008,28(6): 2674–2681.
[20] Guan H L, Chen Y C, Zhu H C, et al. Characteristic study of rhizome-microorganism in soil of diseased panax notoginseng[J]. Agriculture & Technology, 2005, 25(6): 56–58.
[21] Chen Y, Lv G H, Li Y.Soil microbial functional diversity of rhizosphere and non-rhizosphere of three dominant herbaceous plants in the Dushanzi District[J]. Acta Ecologica Sinica, 2018, 38(9): 3110–3117.
[22] Wang X C, Tong X R, Wen X S, et al. Progress on the relationship between plant and rhizospheric microorganism[J]. Shandong Science, 2007, 20(6): 40–44, 50.
[23] Zhou W J, Lv D G, Qin S J.Research progress in interaction between plant and rhizosphere microorganism[J]. Journal of Jilin Agricultural University, 2016, 38(3): 253–260.
[24] Sun L, Ren Z H, Song R J, et al. Isolation, identification and phylogentic analysis of fungal endophyte in Oxytropis trichophra[J]. Acta Veterinaria et Zootechnica Sinica, 2018, 49(9): 2025–2035.
[25] Wen J Z, Lu J Y.Taxonomy and identification of Drechslera,Bipolari and Exserohilum[J]. Journol of Northeast Agricultural College, 1991, 22(2): 120–126.
[26] Peng J H, Lu J Y.Studies on bipolaris, drechslera and exserohilum[J]. Journal of Nanjing Agricultural University, 1989, 12(4): 50–57.
[27] Yao T, Chen Q W, Zhang Y, et al. Analysis of r DNA ITS and carbon metabolic fingerprinting of four Botrytis Nees isolated from three fruits[J]. Journal of Food Science and Technology, 2017, 35(4) :49–55.
[28] Deng Z S, Zhao J F, Lei C, et al. A fungus strain of Botry and its crust effect[J]. Agricultural Research in the Arid Areas, 2012, 30(5): 206–210.
[29] Yang Y L, Liu Y X, Liu Z Y.Research Progress on Fungus Taxonomy of Colletotrichum[J]. Guizhou Agricultural Sciences, 2011, 39(1): 152–157.
[30] Zhang ZY.Plant Pathogenic Mycology [M]. Chengdu: Sichuan Science and Technology Press, 1988.
[31] Zeng S Q, Ling J, Lin L Y, et al. Isolation and identification of a Gladxporism Fungus from mangrove and its cellulase enzyme properties[J]. journal Of Microbiology, 2018, 38(2): 43–48.
[32] Zhai F Y, Liu Y J, Li Y.Notes on new records of Cladosporium, Passalora and Ramularia in inner mongolia, China[J]. Acta Agriculture Boreali-occidentalis Sinica, 2011, 20(4): 5–10.
[33] Ma H M, Liu Y X, Hu L L.Study on biological characteristics of the Aspergillus separated from the roots of Oxyropis glacialis[J]. Journal of Tibet University, 2015, 30(1): 41–44.
[34] Zou X M, Xiao C L, Chen D P, ,et al. Isolation. Isolation and decetion of penicillium in Ginkgo [J]. Journal of Anhui Agriculture Science, 2006, 20: 129, 138.