楚雄地区黄龙尾（Agrimonia pilosa var. nepalensis ）内生菌的分离及初步鉴定

楚雄师范学院学报 ›› 2022, Vol. 37 ›› Issue (3): 33-44.

楚雄地区黄龙尾（Agrimonia pilosa var. nepalensis ）内生菌的分离及初步鉴定

杨海艳¹, 时建玲¹, 陈华红¹, 邱璐^1,*

1.楚雄师范学院资源环境与化学学院, 云南楚雄 675000;
2.楚雄州农业科学院经作所, 云南楚雄 675000

收稿日期:2020-12-03 发布日期:2022-06-30

Isolation and Preliminary Identification of Endophytes of Agrimonia pilosa var. nepalensis in Chuxiong

Haiyan Yang¹, Jianlin Shi¹, Huahong Chen¹, Lu Qiu¹

School of Resources, Environment and Chemistry, Chuxiong Normal University,Yunnan Province 675000

Received:2020-12-03 Published:2022-06-30
Contact: *Lu Qiu （1964–）, female, professor. The research interests include molecules and molecular spectroscopy, E-mail：qiulu@cxtc.edu.cn. Tel. 15887756697
About author:Haiyan Yang（1979－），female，doctor，associate professor，mainly engaged in plant pathology，plant physiology research. E-mail：haiyanyang@cxtc. edu.cn，Tel. 18287864615

摘要/Abstract

摘要： 从野生黄龙尾健康植株内分离得到的29株内生菌中,8株细菌全部为杆菌,有4株产芽孢;10株放线菌,都属于链霉菌属（Streptomyces sp.）;11株真菌,其中7株为镰刀菌属（Fusarium sp.）、2株为炭疽菌属（Colletotrichum sp.）、1株为青霉属（Penicillium sp.）、1株为黑曲霉（ Aspergillus niger）。生长特性实验结果表明,所有供试菌株的最适生长NaCl浓度范围在3%-5%之间,最适生长pH范围在6.0~8.0之间,最适生长温度在20℃~35℃之间,细菌的生长温度范围比放线菌的更为广泛。在生理生化特性实验中,供试菌株B-3-5-4对碳源、氮源的利用范围广,菌株B-3-1-6对碳源、氮源的利用范围窄。研究结果表明,黄龙尾不同组织内部分布着不同类群的内生菌群。本研究可为黄龙尾的进一步开发提供数据支持。

关键词: 黄龙尾, 内生菌, 生长特性, 理化特性

Abstract: 29 endophytes were isolated from healthy plants of wild Agrimonia pilosa var. nepalensis, including 8 strains of bacteria, 10 strains of actinomycetes and 11 strains of fungi. All the bacteria were bacilli and 4 strains produced spores. All actinomycetes belong to Streptomyces sp. . Fungi belong to 4 genera, 7 strains are Fusarium sp., 2 strains are Colletotrichum sp., 1 strain is Penicillium sp., 1 strain is Aspergillus niger. The results of growth characteristics experiment showed that the optimal growth range of all tested strains was 3%~5% NaCl concentration, 6.0~8.0 pH and 20 ℃~35 ℃. The growth temperature range of bacteria was wider than that of actinomycetes. In the experiment of physiological and biochemical characteristics, strain b-3-5-4 used a wide range of carbon and nitrogen sources, while strain b-3-1-6 used a narrow range of carbon and nitrogen sources. The results showed that there were different groups of endophytes in different tissues of Agrimonia pilosa var. nepalensis. This study can provide data for further development of Agrimonia pilosa var. nepalensis.

Key words: Agrimonia pilosa var. nepalensis, endophytes, Growth characteristics, Physical and chemical properties

中图分类号:

Q949.751.8

杨海艳, 时建玲, 陈华红, 邱璐. 楚雄地区黄龙尾（Agrimonia pilosa var. nepalensis ）内生菌的分离及初步鉴定[J]. 楚雄师范学院学报, 2022, 37(3): 33-44.

Haiyan Yang, Jianlin Shi, Huahong Chen, Lu Qiu. Isolation and Preliminary Identification of Endophytes of Agrimonia pilosa var. nepalensis in Chuxiong[J]. Journal of Chuxiong Normal University, 2022, 37(3): 33-44.

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