不同剂量PMSG对云南花兔超数排卵效果的影响

楚雄师范学院学报 ›› 2026, Vol. 41 ›› Issue (3): 16-21.

不同剂量PMSG对云南花兔超数排卵效果的影响

刘骐玮¹, 胡映开², 赵林艳², 严娟³, 朱芳贤⁴, 缪祥虎⁵, 段新慧^1,*, 亐开兴^2,*

1.云南农业大学动物科学技术学院,云南昆明 650201;
2.楚雄师范学院农学院,云南楚雄 675000;
3.云南省农村科技服务中心,云南昆明 650021;
4.全国畜牧总站,北京海淀 100193;
5.曲靖市畜禽改良工作站,云南曲靖 655000

收稿日期:2026-01-13 出版日期:2026-05-20 发布日期:2026-07-22
通讯作者: ^*段新慧（1975-）,女,研究员,研究方向为草产品加工利用;亐开兴（1975-）,男,博士,研究员,研究方向为动物遗传育种。
作者简介:刘骐玮（2000-）,男,硕士研究生,研究方向为动物分子营养与代谢调控。
基金资助:
云南省国际科技特派员专项（No. 202303AK140008）; 云南省“兴滇英才支持计划”产业创新人才培养计划; 楚雄师范学院高原牧业科研团队（No. XJTDA04）; 楚雄师范学院实验室开放项目“云南花兔DNA条形码研究”

The Effect of Different Doses of PMSG on Superovulation in Yunnan Piebold Rabbit

Liu Qiwei¹, Hu Yingkai², Zhao Linyan², Yan Juan³, Zhu Fangxian⁴, Miao Xianghu⁵, Duan Xinhui^1,*, Qu Kaixing^2,*

1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
2. College of Agronomy, Chuxiong Normal University, Chuxiong, Yunnan Province 675000, China;
3. Yunnan Rural Science and Technology Service Center, Kunming, Yunnan Province 650021, China;
4. National Animal Husbandry Service, Beijing 100193, China;
5. Qujing Livestock and Poultry Improvement Station, Qujing, Yunnan Province 655000, China

Received:2026-01-13 Online:2026-05-20 Published:2026-07-22

摘要/Abstract

摘要： 云南花兔处于濒危状态,急需加强高效繁殖及遗传材料收集与保存。本研究用5个不同孕马血清促性腺激素（PMSG）剂量水平（30 g体重/IU、35 g体重/IU、40 g体重/IU、45 g体重/IU和70 g体重/IU,即Ⅱ~Ⅵ组）对云南花兔进行超数排卵处理,与自然发情组（Ⅰ组）对照,观察发情和超数排卵效果。结果表明,高剂量PMSG处理组（Ⅱ组和Ⅲ组）在配种后60~72 h冲胚时血清中仍维持较高的雌二醇（E₂）水平,分别达到（44.18±8.97）pg/mL和（41.45±13.39）pg/mL,显著高于其他四个组的激素水平（P<0.05）;而孕酮（P₄）水平则以第Ⅳ组（40 g体重/IU）的最高,明显高于Ⅰ组和Ⅲ组（P<0.05）,而其他三组间的差异不显著（P>0.05）;高剂量PMSG处理容易引发家兔卵泡充血,Ⅱ组的充血卵泡数量明显增多,显著高于其他四个处理组（P<0.05）;超排效果以Ⅴ组的效果最佳,平均有效胚胎数量为14.90枚,生产中建议使用PMSG 45 g体重/IU剂量+促排3号5 μg进行超数排卵,可取得理想的效果。

关键词: 云南花兔, PMSG, 超数排卵, 胚胎

Abstract: Yunnan Piebold rabbit is an endangered species, which calls for urgent measures to strengthen efficient breeding as well as the collection and preservation of its genetic materials. Compared with natural estrus control （GroupⅠ）, five different doses of pregnant mare serum gonadotropin （PMSG）（30 g body weight/IU, 35 g body weight/IU, 40 g body weight/IU, 45 g body weight/IU and 70 g body weight/IU, i.e. Groups Ⅱ~Ⅵ） were designed for subcutaneous injection to perform superovulation treatment on Yunnan Piebold rabbit for observing the estrus response and the superovulation effect. The results showed that the high-dose PMSG groups maintained a higher E₂ level at 60~72 h of post-copulation to collect embryo, which reached （44.18±8.97）pg/mL for group Ⅱ and （41.45±13.39）pg/mL for group Ⅲ in serum respectively, significantly higher than those of the other four groups （P<0.05）. The P₄ level was highest in group Ⅳ, significantly higher than the corresponding values for groups Ⅰ and Ⅲ （P<0.05）, while there was no significant difference among the other five groups （P>0.05）. The high-dose PMSG treatment groups were also prone to inducing follicular congestion in rabbits, showing the numbers of congestive follicles increase for group Ⅱ, which are significantly higher than those of the other four treatment groups （P<0.05）. The optimal superovulation effect is achieved for group Ⅴ, with effective embryos of 14.90. It is recommended to use PMSG at a dose of 45 g body weight/IU+5 μg luteinizing hormone releasing hormone A3 for superovulation in practice to obtain premium effect.

Key words: Yunnan Piebold rabbit, PMSG, superovulation, embryo

中图分类号:

S829.1

刘骐玮, 胡映开, 赵林艳, 严娟, 朱芳贤, 缪祥虎, 段新慧, 亐开兴. 不同剂量PMSG对云南花兔超数排卵效果的影响[J]. 楚雄师范学院学报, 2026, 41(3): 16-21.

Liu Qiwei, Hu Yingkai, Zhao Linyan, Yan Juan, Zhu Fangxian, Miao Xianghu, Duan Xinhui, Qu Kaixing. The Effect of Different Doses of PMSG on Superovulation in Yunnan Piebold Rabbit[J]. Journal of Chuxiong Normal University, 2026, 41(3): 16-21.