鱼腥草中总黄酮含量测定方法比较研究

楚雄师范学院学报 ›› 2020, Vol. 35 ›› Issue (3): 96-100.

鱼腥草中总黄酮含量测定方法比较研究

李夏冰^*, 江琴, 崔长伟, 李雅善, 王天菊

楚雄师范学院化学与生命科学学院,云南楚雄 675000

收稿日期:2019-11-10 出版日期:2020-05-20 发布日期:2020-12-28
通讯作者: *李夏冰(1987-),男,博士,楚雄师范学院化学与生命科学学院讲师,主要从事天然产物化学研究。E-mail:lixi-abing12345@163.com
基金资助:
云南省教育厅科学研究基金项目资助(NO:2020J0764)

Comparative Study on Assays for Determination of Flavonoids in Houttuynia Cordata

LI Xiabing, JIANG Qin, CUI Changwei, LI Yashan, WANG Tianju

School of Chemistry and Life Sciences,Chuxiong Normal University,Chuxiong,Yunnan Province 675000

Received:2019-11-10 Online:2020-05-20 Published:2020-12-28

摘要/Abstract

摘要： 本研究以芦丁为对照品,比较了直接测定法、AlCl₃法、NaNO₂-Al(NO₃)₃-NaOH法测定鱼腥草中总黄酮的含量,并通过精密度、重现性和加标回收实验进行评价。结果表明:芦丁不适合作为直接测定鱼腥草总黄酮的对照品;NaNO₂-Al(NO₃)₃-NaOH法对鱼腥草总黄酮含量的测定中不具备专一性,受到非黄酮类物质干扰,造成结果偏高。AlCl₃法稳定性、专一性强,可作鱼腥草总黄酮含量的测定方法,其中,AlCl₃法测定波长为 412 nm,精密度、稳定性、重复性的RSD值分别为:1.47％、0.84％、2.03％,加标回收率为94.14±0.28％。

关键词: 鱼腥草, 总黄酮, 分光光度法

Abstract: The accuracy of three spectrophotometric assays for the determination of flavonoids in Houttuynia cordata using direct colorimetry,AlCl₃ and NaNO₂-Al(NO₃)₃-NaOH respectively was compared,and the selected method was evaluated by precision,stability,and recovery test.The results showed1) rutin was not suitable as a standard reference substance for the direct determination of total flavonoids in Houttuynia cordata;and 2) the total flavonoid content measured was higher using NaNO₂-Al(NO₃)₃-NaOH method due to interference with certain non-flavonoids.For the AlCl₃ method,the measurement wavelength was 412 nm using rutin as a reference substance,and AlCl₃ method was selected as the best method for the determination of total flavonoids in Houttuynia cordata.The precision,stability,reproducibility of AlCl₃ method were 1.47%,0.84% and 2.03% respectively.The average recovery rate was 94.14% ±0.28%.

Key words: Houttuynia cordata, total flavonoids, spectrophotometry

中图分类号:

Q949.732.2

李夏冰, 江琴, 崔长伟, 李雅善, 王天菊. 鱼腥草中总黄酮含量测定方法比较研究[J]. 楚雄师范学院学报, 2020, 35(3): 96-100.

LI Xiabing, JIANG Qin, CUI Changwei, LI Yashan, WANG Tianju. Comparative Study on Assays for Determination of Flavonoids in Houttuynia Cordata[J]. Journal of Chuxiong Normal University, 2020, 35(3): 96-100.

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