基于高通量测序的四种落叶果树根际土壤真核生物结构多样性分析

楚雄师范学院学报 ›› 2023, Vol. 38 ›› Issue (3): 38-42.

基于高通量测序的四种落叶果树根际土壤真核生物结构多样性分析

杨贤松

蚌埠学院食品与生物工程学院,安徽蚌埠 233030

收稿日期:2022-05-30 出版日期:2023-05-20 发布日期:2023-08-07
作者简介:杨贤松（1972－），男，博士，教授，研究方向为生物技术。E-mail： yangxs2002@sina.com， Tel：13655527660
基金资助:
安徽省自然科学基金项目（No. 11040606M79）；蚌埠学院自然科学基金项目（No. 2017GJPY07）。

Diversity Analysis of Eukaryotic Structure in RhizoSphere Soil of Four Deciduous Fruit Trees Based on High-throughput Sequencing

YANG Xiansong

College of Food and Bio-engineering, Bengbu University, Bengbu 233030,China

Received:2022-05-30 Online:2023-05-20 Published:2023-08-07

摘要/Abstract

摘要： 为探究四种落叶果树土壤根际真核生物结构多样性情况,对石榴、桃树、樱桃、银杏土壤根际真核生物结构的多样性进行高通量测序和比较分析。结果表明：测序共获得2368个OTUs, 银杏样品的辛普森指数较低,香农指数较高,表明该样品的群落多样性较高,银杏样品的ACE指数和Chaol指数都比其他样品高,表明银杏样品群落丰富度较高。在属水平上,石榴样品真菌群落有四个真菌优势菌属,主要是毛壳菌属（Chaetomium,18.68%）和镰刀菌属（Fusarium,6.77%）。桃树样品真菌群落有五个真菌优势菌属,主要是镰刀菌属（Fusarium,8.87%）和毛壳菌属（Chaetomium,7.00%）。樱桃样品真菌群落有六真菌优势菌属,主要是毛壳菌属（Chaetomium,22.66%）、镰刀菌属（Fusarium,15.91%）及被孢霉属（Mortierella,11.16%）。银杏样品真菌群落有七个真菌优势菌属,主要是毛壳菌属（Chaetomium,9.08%）和Parastagonospora（5.83%）。四种果树样品的优势类群主要是真菌,不同果树样品根际土壤真菌群落差异较大。

关键词: 落叶果树, 高通量测序, 多样性分析

Abstract: The present paper aimed to investigate the diversity of eukaryotic structure in rhizosphere soil of 4 deciduous fruit trees. The diversity of eukaryotic structure in the rhizosphere soil of pomegranate, peach, cherry and ginkgo was sequenced and compared by high-throughput sequencing technique. The results showed that 2368 OTUs were obtained by sequencing. The Shannon index of ginkgo sample was higher and the Simpson index was lower, which indicated that the eukaryotic structure diversity of ginkgo sample was high. The chaol index and ACE index of ginkgo sample were higher than those in the other three samples, which indicated that the richness of eukaryotic community of ginkgo sample was higher. There were four dominant fungal genera in the fungal community of pomegranate sample at the genus level, which mainly included 18.68% of Chaetomium and 6.77% of Fusarium. There were five dominant fungal genera in the fungal community of peach sample at the genus level, which mainly included Fusarium（8.87%） and Chaetomium（7.00%）. There were six dominant fungal genera in the fungal community of cherry sample at the genus level, which mainly included Chaetomium（22.66%）, Fusarium（15.91%） and Mortierella（11.16%）. There were seven dominant fungal genera in the fungal community of ginkgo sample at the genus level, which mainly included Chaetomium（9.08%） and Parastagonospora（5.83%）. The dominant groups of the four fruit samples were fungi, and the fungal communities in rhizosphere soil of different fruit samples were different.

Key words: deciduous fruit trees, high-throughput sequencing, diversity analysis

中图分类号:

S686

杨贤松. 基于高通量测序的四种落叶果树根际土壤真核生物结构多样性分析[J]. 楚雄师范学院学报, 2023, 38(3): 38-42.

YANG Xiansong. Diversity Analysis of Eukaryotic Structure in RhizoSphere Soil of Four Deciduous Fruit Trees Based on High-throughput Sequencing[J]. Journal of Chuxiong Normal University, 2023, 38(3): 38-42.

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