拉曼光谱结合化学计量学方法的复方柳安咖注射液成分分析

楚雄师范学院学报 ›› 2024, Vol. 39 ›› Issue (3): 22-29.

拉曼光谱结合化学计量学方法的复方柳安咖注射液成分分析

张川云^*, 李伦^#, 杨永安, 张德清, 王敏

楚雄师范学院光谱应用技术研究所,云南楚雄 675000

收稿日期:2023-12-30 出版日期:2024-05-20 发布日期:2024-06-05
作者简介:张川云（1990–）,女,讲师,研究方向为分子光谱。E-mail：zcy@cxtc.edu.cn;^#共同第一作者：李伦（1981–）,男,讲师,研究方向为分子光谱。E-mail：liyilun@cxtc.edu.cn
基金资助:
国家自然科学基金项目（No. 11364001）; 云南省教育厅科学研究基金项目（No. 2022J0838）; 云南省科技厅科技计划项目（No. 202101BA070001-187、No. 202301BA070001-058）

Qualitative and Quantitative Analysis of Components in Compound Sodium Salicylate, Antipyrine and Caffeine Injection Based on Raman

ZHANG Chuanyun, LI Lun, YANG Yong'an, ZHANG Deqing, WANG Min

Application Institute of Spectroscopy Technology, Chuxiong Normal University, Chuxiong, Yunnan Province 675000

Received:2023-12-30 Online:2024-05-20 Published:2024-06-05

摘要/Abstract

摘要： 为了实现复方柳安咖注射液成分的快速检测,利用拉曼光谱的指纹识别性、聚焦式采样方法及水影响较低的特点结合化学计量学方法对复方柳安咖注射液进行了分析。复方柳安咖注射液针剂样品的拉曼光谱显示,复方柳安咖注射液针剂的拉曼光谱主要由水杨酸钠、安替比林和咖啡因的水溶液拉曼谱带组成。以水杨酸钠梯度溶液拉曼光谱特征峰812 cm^-1和1033 cm^-1、安替比林梯度溶液拉曼光谱特征峰724 cm^-1和1005 cm^-1,依据散射光强与相应组分浓度之间的关系,利用光谱特征峰高度、峰面积分别建立定量分析模型;考虑各成分光谱间的相互干扰,也利用水杨酸钠梯度溶液光谱745~920 cm^-1、1010~1060 cm^-1波段数据和安替比林梯度溶液拉曼光谱700~750 cm^-1、950~1020 cm^-1波段数据,基于偏最小二乘法（PLS）建立定量分析模型。两种分析模型对三个不同厂家不同生产批次的31个针剂样品中主要成分水杨酸钠和安替比林的含量进行预测,预测值与液相色谱法测试值相近;预测水杨酸钠和安替比林含量分别为标示量的96.4% ~102.3%之间和98.1% ~ 105.8%之间,都符合国家药品标准,与液相色谱测试法的分析结果一致。表明拉曼光谱结合化学计量学方法能对复方柳安咖注射液成分进行定量分析,具有准确、方便、快捷的优点。

关键词: 拉曼光谱, 复方柳安咖注射液, 化学计量学, 定量分析

Abstract: In order to achieve the rapid detection of the effective components in the Compound Sodium Salicylate, Antipyrine and Caffeine Injection, this study focuses on utilizing the Raman spectroscopy technique with its superiority of low water interference, fingerprint recognition and focused sampling. Raman spectra of the Compound Sodium Salicylate, Antipyrine and Caffeine Injection samples were analyzed, and the results showed that the Raman spectra of injection samples were mainly composed of the Raman spectra of sodium salicylate aqueous solution, antipyrine aqueous solution and caffeine aqueous solution. According to the relationship between the Raman scattering intensity and the concentration of the corresponding component, the quantitative analysis models using the spectral characteristic peaks height or peaks area were established based on the spectral characteristic peaks at 812 cm^-1 and 1033 cm^-1 of sodium salicylate gradient solution, and the spectral characteristic peaks at 724 cm^-1 and 1005 cm^-1 of antipyrine gradient solution, respectively. Considering the mutual interference between the spectra of each component, the quantitative analysis models based on partial least squares (PLS) were established by using the spectral data of sodium salicylate gradient solution in the 745~920 cm^-1 and 1010~1060 cm^-1 region, and the spectral data of antipyrine gradient solution in the 700~750 cm^-1 and 950~1020 cm^-1 region, respectively. Then, the contents of sodium salicylate and antipyrine in 31 injection samples from different production batches of three different manufacturers were predicted by the two kinds of quantitative analysis models, and the predicted values were compared with those measured by liquid chromatography. The results showed that the predicted values were close to the measured values of liquid chromatography. Among the 31 injection samples, the predicted content of sodium salicylate was between 96.4% and 102.3% of the labeled amount, and the predicted content of antipyrine was between 98.1% and 105.8% of the labeled amount. The results all met the national drug standards. The analysis result was also consistent with the result of liquid chromatography. It is indicated that the Raman technique can be utilized as the qualitative and quantitative analysis of the components of Compound Sodium Salicylate, Antipyrine and Caffeine Injection.

Key words: Raman spectrum, compound sodium Salicylate Antipyrine and Caffeine Injection, qualitative analysis, quantitative analysis

中图分类号:

R971.1

张川云, 李伦, 杨永安, 张德清, 王敏. 拉曼光谱结合化学计量学方法的复方柳安咖注射液成分分析[J]. 楚雄师范学院学报, 2024, 39(3): 22-29.

ZHANG Chuanyun, LI Lun, YANG Yong'an, ZHANG Deqing, WANG Min. Qualitative and Quantitative Analysis of Components in Compound Sodium Salicylate, Antipyrine and Caffeine Injection Based on Raman[J]. Journal of Chuxiong Normal University, 2024, 39(3): 22-29.

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