节节草中黄酮的提取及其抗氧化性研究

楚雄师范学院学报 ›› 2020, Vol. 35 ›› Issue (3): 86-90.

节节草中黄酮的提取及其抗氧化性研究

江岸, 张伦, 杨申明, 张晶

楚雄师范学院化学与生命科学学院,云南楚雄 675000

收稿日期:2019-11-20 出版日期:2020-05-20 发布日期:2020-12-28
作者简介:江岸(1974-),女,博士,楚雄师范学院化学与生命科学学院讲师,主要研究方向为植物有效成分的提取和应用。E-mail:jianganhust@cxtc.edu.cn
基金资助:
国家自然科学基金(NO:11364001)

Study on Extraction and Antioxidant Activity of Flavonoids from Equisetum Ramosissimum

JIANG An, ZHANG Lun, YANG Shenming, ZHANG Jing

School of Chemistry and Life Sciences,Chuxiong Normal University,Chuxiong,Yunnan Province 675000

Received:2019-11-20 Online:2020-05-20 Published:2020-12-28

摘要/Abstract

摘要： 本文以乙醇为溶剂,用微波法提取节节草中的黄酮,通过单因素实验研究乙醇体积分数、微波功率、料液比和加热时间对提取率的影响,利用L₉(3⁴)正交试验得出最佳提取条件:乙醇体积分数80%,微波功率210 W,料液比为1:30(g/mL),加热时间90 s,在此条件下黄酮的提取率为9.9 mg/g。节节草黄酮提取液对超氧阴离子自由基(O^2-·)和羟基自由基(·OH)的清除率分别达到28.59%和41.27%,表明节节草黄酮提取液具有一定的抗氧化活性。

关键词: 节节草, 微波, 黄酮, 抗氧化性

Abstract: In this paper,flavonoid has been extracted from Equisetum ramosissimum Desf.by microwave assisted method with ethanol.The effect of ethanol concentration,microwave power,solid-liquid ratio and extraction time has been studied with single-factor experimental method.Results of L₉ (3⁴) orthogonal experiment show that the optimum extraction parameters areethanol concentration 80%,microwave power 210 W,solid-liquid ratio 1:30 (g/mL),and extraction time 90 s.Under these conditions,the extraction rate is 9.9 mg/g,the percentage scavenging of O^2-·and ·OH,are 28.59% and 41.27%,implying that these flavonoids have good ability to scavenge ·OH,O^2-·.

Key words: Equisetum ramosissimum, microwave, flavonoids, antioxidant properties

中图分类号:

TQ253.1

江岸, 张伦, 杨申明, 张晶. 节节草中黄酮的提取及其抗氧化性研究[J]. 楚雄师范学院学报, 2020, 35(3): 86-90.

JIANG An, ZHANG Lun, YANG Shenming, ZHANG Jing. Study on Extraction and Antioxidant Activity of Flavonoids from Equisetum Ramosissimum[J]. Journal of Chuxiong Normal University, 2020, 35(3): 86-90.

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