三白草科质体基因组比较研究

楚雄师范学院学报 ›› 2025, Vol. 40 ›› Issue (3): 93-99.

三白草科质体基因组比较研究

刘常坤, 邵曼, 陈姝黎, 邱聃, 胥祥翠, 班鑫瑶

楚雄师范学院资源环境与化学学院,云南楚雄 675000

收稿日期:2025-02-12 出版日期:2025-05-20 发布日期:2025-07-01
作者简介:刘常坤（1994–）,男,讲师,研究方向为植物系统分类。
基金资助:
云南省教育厅科学研究基金项目（No.2024J0988）; 楚雄师范学院博士科研启动项目（No.BSQD2311）

The Plastome Comparison of Saururaceae

LIU Changkun, SHAO Man, CHEN Shuli, QIU Dan, XU Xiangcui, BAN Xinyao

School of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong, Yunnan Province 675000

Received:2025-02-12 Online:2025-05-20 Published:2025-07-01

摘要/Abstract

摘要： 三白草科是一个具有重要药用价值的残存古草本类群,呈典型的东亚–北美间断分布。但该科植物的质体基因组特征尚不明确,这严重影响了三白草科植物资源的开发利用以及我们对被子植物起源、早期演化和地理分布式样成因的理解。本研究对三白草科4属中3属植物进行系统、全面的质体基因组比较分析,结果表明三白草科质体基因组在基因组结构、基因数量和排列方面高度保守,但基因组长度存在明显差异,这可能由碱基的插入或缺失造成。此外,我们挑选出2个高变片段（rps16-trnQ-UUG和rpl 32-trnL-UAG）作为三白草科植物的候选专属条形码,并提出三核苷酸、五核苷酸和六核苷酸重复可以作为识别三白草的特定分子标记。总之,本研究为深入理解三白草科植物演化和物种识别提供了新视角。

关键词: 三白草科, 质体基因组, 简单重复序列, 高变片段

Abstract: The Saururaceae family, a member of remaining paleoherbs with medicinal value, shows typically East Asian-North American disjunction. However, plastome features of this family have not been clarified, which has severely hindered the utilization of Saururaceae plants and understanding of the origin, early evolution and distribution pattern of angiosperm. Hence, we conducted comprehensively plastome comparative analyses for Saururaceae, including three genera of four genera. The results showed that plastomes of Saururaceae were highly conserved in terms of genomic structure, gene content and arrangement. Nevertheless, their genomic sizes varied remarkably, which may be caused by the insertion/deletion of bases. In addition, two high variable regions (rps16-trn Q-UUG and rpl 32-trn L-UAG) were selected among plastomes that can serve as candidate specific barcoding for species identification of Saururaceae and three SSR types (tri-nucleotides, penta-nucleotides and hexa-nucleotides) were provided as specific molecular makers to recognize Saururus chinensis. Generally speaking, our results provide new insights into species evolution and identification of Saururaceae.

Key words: Saururaceae, plastome comparison, simple sequence repeat, high variable fragment selecting

中图分类号:

S567.239

刘常坤, 邵曼, 陈姝黎, 邱聃, 胥祥翠, 班鑫瑶. 三白草科质体基因组比较研究[J]. 楚雄师范学院学报, 2025, 40(3): 93-99.

LIU Changkun, SHAO Man, CHEN Shuli, QIU Dan, XU Xiangcui, BAN Xinyao. The Plastome Comparison of Saururaceae[J]. Journal of Chuxiong Normal University, 2025, 40(3): 93-99.

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