Isolation and Preliminary Identification of Endophytes of Agrimonia pilosa var. nepalensis in Chuxiong

Journal of Chuxiong Normal University ›› 2022, Vol. 37 ›› Issue (3): 33-44.

• Life Science & Chemistry • Previous Articles Next Articles

Isolation and Preliminary Identification of Endophytes of Agrimonia pilosa var. nepalensis in Chuxiong

Haiyan Yang¹, Jianlin Shi¹, Huahong Chen¹, Lu Qiu¹

School of Resources, Environment and Chemistry, Chuxiong Normal University,Yunnan Province 675000

Received:2020-12-03 Published:2022-06-30
Contact: *Lu Qiu （1964–）, female, professor. The research interests include molecules and molecular spectroscopy, E-mail：qiulu@cxtc.edu.cn. Tel. 15887756697
About author:Haiyan Yang（1979－），female，doctor，associate professor，mainly engaged in plant pathology，plant physiology research. E-mail：haiyanyang@cxtc. edu.cn，Tel. 18287864615

Abstract

Abstract: 29 endophytes were isolated from healthy plants of wild Agrimonia pilosa var. nepalensis, including 8 strains of bacteria, 10 strains of actinomycetes and 11 strains of fungi. All the bacteria were bacilli and 4 strains produced spores. All actinomycetes belong to Streptomyces sp. . Fungi belong to 4 genera, 7 strains are Fusarium sp., 2 strains are Colletotrichum sp., 1 strain is Penicillium sp., 1 strain is Aspergillus niger. The results of growth characteristics experiment showed that the optimal growth range of all tested strains was 3%~5% NaCl concentration, 6.0~8.0 pH and 20 ℃~35 ℃. The growth temperature range of bacteria was wider than that of actinomycetes. In the experiment of physiological and biochemical characteristics, strain b-3-5-4 used a wide range of carbon and nitrogen sources, while strain b-3-1-6 used a narrow range of carbon and nitrogen sources. The results showed that there were different groups of endophytes in different tissues of Agrimonia pilosa var. nepalensis. This study can provide data for further development of Agrimonia pilosa var. nepalensis.

Key words: Agrimonia pilosa var. nepalensis, endophytes, Growth characteristics, Physical and chemical properties

CLC Number:

Q949.751.8

Haiyan Yang, Jianlin Shi, Huahong Chen, Lu Qiu. Isolation and Preliminary Identification of Endophytes of Agrimonia pilosa var. nepalensis in Chuxiong[J]. Journal of Chuxiong Normal University, 2022, 37(3): 33-44.

References

[1] Editorial board of flora of China, Chinese Academy of Sciences. Flora of China [M]. Beijing:Science Press, 1978,37:458
[2] Ren J Y, Zhang J, Zhang Q B.Comparison research of herbs of Agrimonia pilosa var. nepalensis and Agrimonia pilosa Ledeb[J]. Chinese Journal of Pharmaceutical Analysis, 2007,27(11):1728-1731.
[3] Kunming Institute of Botany, Chinese Academy of Sciences. Flora of Yunnan (Vol.12) [M]. Beijing:Science Press, 2003:600-603.
[4] Jin M,Liu S Y.Research Progress on the Agrimonia pilosa Ledeb[J]. Journal of Anhui Agriculture Science, 2015,43(19):78-79,115.
[5] Jiang W H, Wang X D, Jin Y J.Clinical application progress of Agrimonia pilosa[J]. World's Latest Medical Information Abstracts: Electronic Edition, 2013, 000(011):321-321
[6] Song J.The study on evaluation of in vitro coagulation effects and antibacterial of Chinese herbals [D]. Nanning: Guangxi University, 2016,6:1-57.
[7] Cong Y X.Clinical observation and nursing of 50 cases of upper gastrointestinal bleeding treated with large dose of Agrimonia pilosa[J]. Journal of Qilu Nursing, 2014,20(17):77-78.
[8] Wang D Y,Xu G M,Li Y.Experience of Professor Li Siwen in the treatment of tumor by using agrimonia herb[J]. Guangming Journal of Chinese Medicine, 2016,31(21):3096-3097.
[9] Wang L, Zhang R Q.Research overview of different extracts of Agrimonia pilosa on tumor cells[J]. Tianjin Pharmacy, 2016,28(4):46-48.
[10] Yang Y.Chemical constituents of Taxus yunnanensis, Agrimonia pilosa var. nepalensis and Crepis napifera [D]. Kunming: Yunnan University of Traditional Chinese Medicine, 2014,6:1-140.
[11] Shi Y W, Lou K, Li C.The application of endophytic microbes in bio-control[J]. Journal of Microbiology, 2009,29(6):61-64.
[12] Wang Z Y, Liu X J.Research status on separation methods of plant endophytes[J].Guizhou Agricultural Sciences, 2014,42(1):152-155.
[13] Ma L J, Wang J Y, Zhang Y L, et al. The Bioactive diversity of endophytes secondary metabolites and application prospects[J]. Journal of Shenyang Normal University (Natural Science Edition), 2017,35(3):344-348.
[14] Hasegawa H, Meguro A, Shimizu M, et al. Endophytic antinomycetes and their interactions with host plants[J]. Actinomycetologica, 2006,20(2):72-81.
[15] Sturz A V, Christie B R, Norwak J.Bacterial endophytes:potential role in developing sustainable systems of crop porduction[J].Critical Reviews in Plant Sciences, 2000,19(1):1-30.
[16] Chen M L, Huang L Q, Ou Yang S H, et al. Effect of endophytes on the formation of genuine medicinal materials[J]. Chinese Journal of Information on TCM, 2006,13:40-41.
[17] Gong A D, Han M Z, Kong X W, et al. Application analysis of endophytic microbes in Camellia sinensis[J]. Journal of Xinyang Normal University Natural Science Edition, 2017,30(1):168-172.
[18] Liu J H,Wu L F,Zhang H W.Isolation and preliminary identification of endophytes from Artemisia annua Linn[J]. Amino Acids ＆ Biotic Resources, 2011,33(4):27-30.
[19] Shi J Y, Chen W X, Liu A Y.Advances in the study of endophytes and their effects on control of plant diseases[J]. Acta Ecologica Sinica, 2006,26(7):2395-2401.
[20] Yang B S, Jiang G Y, Liu J, et al. Development of Yunnan’s medicinal plant resources and the research of endophytes[J]. Journal of Anhui Agriculture Science, 2011,39(28):17228-17229,17231.
[21] Peng Z Y.Study on antitumor constituents of metabolites of Endophytic Fungi from Agrimonia pilosa[J]. World Health Diges, 2008,5(8):998-999.
[22] Ding W J, Wang S S, Ren J Q, et al. Progress on plant endophyte[J]. Current Biotechnology, 2015,5(6):425-428.
[23] Yang H Y, Li Y S, Li R J, et al. Preliminary study on the endophytes and rhizosphere soil microbes of Cyclobalanopsis glaucoides[J]. Journal of Chuxiong Normal University, 2021,36(03):67-79.
[24] Chen H, Hong K, Zhuang L, et al. Growth characteristics and fermentation condition optimization of Mangrove Actinamycete strain 0616167[J]. Microbiology China, 2006,3(2):16-20.
[25] Wang B Q, Jin Z X.Research progress on constituents and antioxidant in Agrimonia pilosa Ledeb[J]. Northern Horticulture, 2011(10):167-169.
[26] Yu H N, Yao Z, Shen S R.Distribution of endophytic fungi in Agrimonia pilosa Ledeb from different areas and screening of active strains[J]. Journal of Zhejiang Unverisity of Technology, 2013,4(3):260-264.
[27] Feng T X, Lu K Y, Lu L Y, et al. Research progress in diversity of endophytic actinomycetes[J]. Journal of Microbiology, 2015,35(3):97-104.
[28] Chen X X, Yu H N, Ying Y M, et al. Isolation and identification of endophytic fungi from Agrimonia pilosa Ledeb and pilot study of its antibacterial activity[J], Journal of Zhejiang University of Technology, 2011,39(1):39-43.
[29] Li L M, Bai R Q, Wang Y C.Changes of physiological and biochemical in NaCl stress of Agrimonia pilosa Ledeb[J], Journal of Inner Mongolia Agricultural University, 2011,32(4):121-123.
[30] Li L M, Bai R Q, Li Y, et al. Effects of salt stress on the growth、leaf cat activity and chlorophyll content in wild plant Agrimonia pilosa Ledeb[J].Journal of Inner Mongolia Agricultural University, 2010,31(4):77-80.

Isolation and Preliminary Identification of Endophytes of Agrimonia pilosa var. nepalensis in Chuxiong

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