Journal of Chuxiong Normal University ›› 2020, Vol. 35 ›› Issue (3): 96-100.

• Chemistry • Previous Articles     Next Articles

Comparative Study on Assays for Determination of Flavonoids in Houttuynia Cordata

LI Xiabing, JIANG Qin, CUI Changwei, LI Yashan, WANG Tianju   

  1. School of Chemistry and Life Sciences,Chuxiong Normal University,Chuxiong,Yunnan Province 675000
  • Received:2019-11-10 Online:2020-05-20 Published:2020-12-28

Abstract: The accuracy of three spectrophotometric assays for the determination of flavonoids in Houttuynia cordata using direct colorimetry,AlCl3 and NaNO2-Al(NO3)3-NaOH respectively was compared,and the selected method was evaluated by precision,stability,and recovery test.The results showed1) rutin was not suitable as a standard reference substance for the direct determination of total flavonoids in Houttuynia cordata;and 2) the total flavonoid content measured was higher using NaNO2-Al(NO3)3-NaOH method due to interference with certain non-flavonoids.For the AlCl3 method,the measurement wavelength was 412 nm using rutin as a reference substance,and AlCl3 method was selected as the best method for the determination of total flavonoids in Houttuynia cordata.The precision,stability,reproducibility of AlCl3 method were 1.47%,0.84% and 2.03% respectively.The average recovery rate was 94.14% ±0.28%.

Key words: Houttuynia cordata, total flavonoids, spectrophotometry

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