EST-SSR Sequence Analysis of Genus Camellia

Journal of Chuxiong Normal University ›› 2021, Vol. 36 ›› Issue (3): 39-50.

• Biology • Previous Articles Next Articles

EST-SSR Sequence Analysis of Genus Camellia

Cunli Dong^1,#, Guoyan Kou^2,#, Chunmei Luo^3,#, Yan Zhang^4,#, Haiyan Yang^2,*, Shuguo Fan², Qianjin Chen⁵, Jiangong Yu⁵, Shengfu Shao⁶, Changjie Jiang⁷, Yangying Luo⁷, Lu Qiu^2,*

1.Chuxiong Administration, Ailao Mountain National Nature Reserve of Yunnan, Chuxiong 675000, China;
2. School of Resources, Environment & Chemistry, Chuxiong Normal University, Chuxiong, Yunnan Province 675000;
3. Department of Modern Landscape Engineering,Chuxiong Technician College,Chuxiong,Yunnan 675000,China;
4. Institute of Cash Crops, Chuxiong Academy of Agricultural Sciences, Chuxiong 675000, China;
5. Forestry Seedling Station of Jinhua City, Jinhua 321000, China;
6. Jinhua International Camellia Species Park, Jinhua 321000, China;
7. Golden Flower Tea Park, Guangxi Province, Nanning 530000, China

Received:2019-11-02 Online:2021-05-20 Published:2021-07-23
Contact: *HaiyanYang（1979–）, doctor, associate professor, mainly engaged in plomt pathology, plomtphysiology reserch, E-mail：haiyanyang@cxtc.edu.cn; Lu Qiu（1964–）, professor, mainly engaged in DNA and RNA research, E-mail ： qiulu@cxtc.edu.cn, Tel. 0086-8783120042
About author:#Cunli Dong, senior engineer, mainly engaged in cultivation and protection of forest resources, E-mail： 2824067245@qq.com; Guoyan Kou, mainly engaged in DNA analysis, E-mail：171123518@qq.com; Chunmei Luo, associate professor, mainly engaged in crop growing, E-mail：546367998@qq.com; Yan Zhang, senior researcher, mainly engaged in the research of cash crops, E-mail：2929274868

Abstract

Abstract: 179 plants of Camellia genus were analyzed using SSR, EST-SSR molecular markers technology to detect their genetic relationship. DNAs of the 179 Camellia plants were extracted by improved CTAB, then tested for the quality of DNAs by way of 1 percent of agarose gel electrophoresis, and tested for PCR amplification by way of 2 percent of agarose gel electrophoresis. The results of amplification products show, the EST-SSR sequence fragment size is 194bp, the longest being Chongzhu （C. theopsis） of 210bp and shortest being Aomai （C. impressinervis） of 152bp. Referring to the homologous series in NCBI and compared using Cluster X software, great differences were found in the EST-SSRs of different camellia plants, with 1752 deletion loci and 813 mutation sites. Phylogenetic trees were constructed using MEGA 6.0 to analyze their genetic relationship, 39 camellia plants were classified into four groups, i.e. Sect. Camellia, Sect. Chrysantha, Sect. Furfuracea and Sect. Theopsis. The results are basically in consistency with Zhang Hongda morphological classification. This indicates that EST-SSR molecular marker is effective in phylogenetic classification and identification of Camellia.

Key words: Genus Camellia, SSR, EST-SSR, phylogenetic tree, genetic relationship

CLC Number:

Q949.758.4

Cunli Dong, Guoyan Kou, Chunmei Luo, Yan Zhang, Haiyan Yang, Shuguo Fan, Qianjin Chen, Jiangong Yu, Shengfu Shao, Changjie Jiang, Yangying Luo, Lu Qiu. EST-SSR Sequence Analysis of Genus Camellia[J]. Journal of Chuxiong Normal University, 2021, 36(3): 39-50.

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EST-SSR Sequence Analysis of Genus Camellia

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