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EST-SSR Sequence Analysis of Genus Camellia
Cunli Dong, Guoyan Kou, Chunmei Luo, Yan Zhang, Haiyan Yang, Shuguo Fan, Qianjin Chen, Jiangong Yu, Shengfu Shao, Changjie Jiang, Yangying Luo, Lu Qiu
2021, 36(3):
39-50.
179 plants of Camellia genus were analyzed using SSR, EST-SSR molecular markers technology to detect their genetic relationship. DNAs of the 179 Camellia plants were extracted by improved CTAB, then tested for the quality of DNAs by way of 1 percent of agarose gel electrophoresis, and tested for PCR amplification by way of 2 percent of agarose gel electrophoresis. The results of amplification products show, the EST-SSR sequence fragment size is 194bp, the longest being Chongzhu (C. theopsis) of 210bp and shortest being Aomai (C. impressinervis) of 152bp. Referring to the homologous series in NCBI and compared using Cluster X software, great differences were found in the EST-SSRs of different camellia plants, with 1752 deletion loci and 813 mutation sites. Phylogenetic trees were constructed using MEGA 6.0 to analyze their genetic relationship, 39 camellia plants were classified into four groups, i.e. Sect. Camellia, Sect. Chrysantha, Sect. Furfuracea and Sect. Theopsis. The results are basically in consistency with Zhang Hongda morphological classification. This indicates that EST-SSR molecular marker is effective in phylogenetic classification and identification of Camellia.
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