一株杜鹃兰（Cremastra appendiculata）内生细菌的分离及全基因组分析

楚雄师范学院学报 ›› 2026, Vol. 41 ›› Issue (3): 112-119.

一株杜鹃兰（Cremastra appendiculata）内生细菌的分离及全基因组分析

李光富¹, 张明¹, 陈华红^2,*

1.楚雄医药高等专科学校基础医学院,云南楚雄 675000;
2. 楚雄师范学院农学院,云南楚雄 675000

收稿日期:2025-10-16 出版日期:2026-05-20 发布日期:2026-07-22
通讯作者: ^*陈华红（1971-）,女,博士,教授,研究方向为微生物资源。
作者简介:李光富（1967-）,男,副教授,研究方向为生理学。
基金资助:
云南省教育厅科学研究基金项目（No. 2021J1121）; 楚雄州专家工作站建设项目（No. 20242305A020003）

Isolation and Whole-Genome Analysis of an Endophytic Bacterium from Cremastra Appendiculata

Li Guangfu¹, Zhang Ming¹, Chen Huahong^2,*

1. School of Basic Medical Sciences, Chuxiong Medical College, Chuxiong, Yunnan Province 675000, China;
2. College of Agronomy, Chuxiong Normal University, Chuxiong, Yunnan Province 675000, China

Received:2025-10-16 Online:2026-05-20 Published:2026-07-22

摘要/Abstract

摘要： 为了解杜鹃兰假鳞茎病害相关内生细菌及其潜在宿主互作基因特征,从云南楚雄杜鹃兰假鳞茎的患病部位分离得到一株内生细菌,通过表型特征、16S rDNA序列及全基因组序列分析确定了内生菌的分类地位。结果表明：从杜鹃兰患病组织分离的菌株N-EI为Brevibacterium sediminis。通过菌株N-EI的全基因组序列分析,菌株N-EI 基因组大小为 4299738 bp,共预测得到3817条基因,在菌株N-EI的基因组中共存在58个常见的毒力因子相关基因,211个植物互作相关基因,这些基因可能与菌株在宿主组织中的定殖、环境适应及假鳞茎病害相关过程有关。

关键词: 杜鹃兰, 假鳞茎, 内生细菌, 全基因组分析

Abstract: To investigate bacteria associated with pseudobulb disease of Cremastra appendiculata and the genetic characteristics of their potential interaction with the host, an endophytic bacterial strain was isolated from diseased pseudobulb tissues collected in Chuxiong, Yunnan Province. The taxonomic status of this endophyte was determined through phenotypic characterization, 16S rDNA sequencing and whole-genome sequence analysis. The results showed that the strain N-EI, isolated from the diseased C. appendiculata tissue, is Brevibacterium sediminis. Whole-genome sequencing revealed that strain N-EI has a genome size of 4299738 bp, with a total of 3817 predicted genes. Notably, the genome of strain N-EI harbors 58 common virulence factor-associated genes and 211 plant interaction-related genes. The synergistic effects of these genes may be associated with the pathogenesis of the pseudobulb disease.

Key words: Cremastra appendiculata, pseudobulb, endophytic bacterium, whole-genome analysis

中图分类号:

S436.639

李光富, 张明, 陈华红. 一株杜鹃兰（Cremastra appendiculata）内生细菌的分离及全基因组分析[J]. 楚雄师范学院学报, 2026, 41(3): 112-119.

Li Guangfu, Zhang Ming, Chen Huahong. Isolation and Whole-Genome Analysis of an Endophytic Bacterium from Cremastra Appendiculata[J]. Journal of Chuxiong Normal University, 2026, 41(3): 112-119.