Bioinformatics Analysis of Bovine Insulin-like Growth Factor-1 Receptor

Journal of Chuxiong Normal University ›› 2020, Vol. 35 ›› Issue (6): 76-88.

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Bioinformatics Analysis of Bovine Insulin-like Growth Factor-1 Receptor

Haiyan Yang¹, Huanfei Kuang¹, Ruijun Li², Zhongze Luo³, Lu Qiu^1,*

1. Department of Chemistry, Life Science, Chuxiong Normal University, Chuxiong675000;
2. College of Foreign Languages, Literature, Chuxiong Normal University, Chuxiong675000;
3. Chuxiong Agricultural Science Research Institute, Yunnan Chuxiong675000

Received:2020-03-28 Online:2020-11-20 Published:2021-03-29
Contact: *Lu Qiu （1964–）, Female, master, professor, mainly engaged in Biomolecules and molecular spectra. E-mail： qiulu@cxtc.edu.cn, Tel：18908787795
About author:Haiyan Yang（1979－）,Female,doctor,associate professor,mainly engaged in plant pathology,physiology and edible fungi research. E-mail：haiyanyang@cxtc.edu.cn，Tel：18287864615;#Co-first author：Huanfei Kuang（1997－）,Female,undergraduate,mainly engaged in biological research. E-mail：3345034225@qq.com，Tel：13176655136

Abstract

Abstract: In order to understand the gene and protein structure of Insulin-like growth factor-1 receptor in Bos taurus and other animals, to know the differences between the various structures of IGF-1R and the corresponding functions of these animals, in this paper, the protein and gene sequences of 9 species of IGF-1R mammals in the NCBI database including B. taurus, Maylandia zebra, Capra hircus, Bubalus bubalis, Ovis aries, Sus scrofa, Equus caballus, Camelus dromedaries and Homo sapiens was used as research objects. Using the relevant software and website of bioinformatics, the correlation between the composition structure and function of the gene sequence and protein sequence of IGF-1R of these 9 animals is comprehensively analyzed. The results of the study indicate the IGF-1R gene sequences of that some of the 9 animal have promoters and tailing sites, and some do not exist; all contain open reading frames and can encode complete polypeptide chains. The amino acids in the protein sequences of 9 animal IGF-1Rs are acidic amino acids, and not all of them have signal peptides; except for the B. bubalis, the identity and similarity of the protein sequence of bovine IGF-1R with other animals is between 25~35; the evolutionary trees constructed by multiple sequences have differences, one is the difference in evolutionary years, and the other is that the IGF-1R protein sequences of these animals have homology; The secondary structure is mainly composed of random coils; the tertiary structure of bovine IGF-1R protein sequence is quite different from other animals, but the structure is relatively stable. The predicted model conformation of the tertiary structure of IGF-1R protein sequence of 9 animals is in accordance with the rules of stereochemistry. The research results have a better understanding of the structure and function of bovine IGF-1R, and provide a certain reference for further discussion on the structure and function of IGF-1R, animal growth and reproduction.

Key words: Bos Taurus, Insulin-like growth factor-1 receptor, Bioinformatics analysis, Protein sequence, Gene sequence

CLC Number:

Q578

Haiyan Yang , Huanfei Kuang , Ruijun Li, Zhongze Luo , Lu Qiu. Bioinformatics Analysis of Bovine Insulin-like Growth Factor-1 Receptor[J]. Journal of Chuxiong Normal University, 2020, 35(6): 76-88.

References

[1] Chen S Q,Li J X,Wu X Y,et al.Progress in molecular mechanisms of insulin resistance[J].Journal of Liaoning University of Traditional Chinese Medicine,2020,22(2):87–92.
[2] Yang B,Xue H B,Wang F.Research status and progress of insulin-like growth factor-1[J].Advances in Clinical Medicine,2019,9(7):827–830.
[3] Li S X,Li J Q,Tian Y B,et al. Differential tissue expression of porcine IGF-1R,cloning and genetic polymorphisms of the 5’regulatory region[J].Acta Agriculture Universitatis Jianxiensis,2013,35(4):813–818,825.
[4] Zou X L,Ye R R,Wang Y,et al.Research progress of insulin-like growth factor-1 and its receptor in tumor[J].Chongqing Medicine,2018,47(18):2474–2476.
[5] Zhu L Q,Ding Z K,Li X L,et al.Application progress of insulin-like growth factor type 1 receptor in molecular imaging[J].Biomedical Engineering and Clinical Medicine,2020,24(1):98–104.
[6] Li X L,Li J Y.Progress in study on relationship between insulin-like growth factor-1 receptor and tumor[J].Foreign Medical(Section of Maternal and Child Health),2005,16(6):435–437.
[7] Wang Y C,Qi W Q,Zhao P.Mechanism and progress of insulin-like growth factor 1 receptor in the treatment of pancreatic cancer[J].Journal of Clinical Hepatology,2017,33(4):790–794.
[8] Ji Y Y,Wang Z D,Wang B T.IGF-1R blocker inhibits the proliferation and metastasis of human liver cancer cell MHCC97-H[J].Modern Oncology Medicine,2016,24(5):681–685.
[9] Tang M,Pan H,Huang Y Y,et al.Serum levels and clinical significance of IGF-1,IGFBP-4 and PAPPA in non-small cell lung cancer[J].China oncology,2015,25(6):439–445.
[10] Liu Y M,Ma S J,Chen M W,et al.clinical significance and expression of insulin-like growth factor-1 receptor (IGF-1R) in non-small cell lung cancer tissues[J].Modern Oncology,2019,27(14):2505–2509.
[11] Yu Z H,Wang X X,Shi Y Q,et al.Expression of IGF-IR and SUSD3 in breast cancer and their clinical significance[J].Chinese Clinical Oncology,2014,19(4):303–306.
[12] Mei X L,Li C Y,Kuang L D,et al.Research progress on the relationship between insulin-like growth factor 1 receptor polymorphism and production performance[J].Jiangsu Agricultural Sciences,2019,47(2):29–32.
[13] Stanilova S A,Ivanova M G,Karakolev I A,et al.Association of +3179G/A insulin-like growth factor-1 receptor polymorphism and insulin-like growth factor-1 serum level with systemic lupus erythematosus[J].Lupus,2013,22(13):1388–1393.
[14] Wang J,Song J Q,Meng Z D.Study on the relationship between insulin-like growth factor-1 receptor gene polymorphism and systemic lupus erythematosus[J].Medical Journal of Wuhan University,2015,(2):285–288.
[15] Jia B,Xi J F,Zhang Y,et al.Developmental changes and breed characteristics of GHR,IGF-1 and IGF-1R gene expression in sheep skin[J].Genetic,2006,(9):1078–1082.
[16] Szewczuk M,Zych S,Wójcik J,et al.Association of two SNPs in the coding region of the insulin-like growth factor 1 receptor(IGF-1R) gene with growth-related traits in Angus cattle[J].Journal of Applied Genetics,2013,54(3):305–308.
[17] Wang S Y.Biological sequence similarity analysis and gene recognition based on signal processing technology [D].Chongqing:Chongqing University,2011.
[18] Yuan X L,Zhou J,Xin P Y,et al.Bioinformatics analysis of CHI genes in eight plant species[J].Journal of Southwest Forestry University,2013,33(2):88–95.
[19] Mai X Y,Li P,Xu L.Bioinformatics analysis of sequence and structure of insulin-like growth factor-1 receptor[J].Chinese Journal of Biotechnology,2016,32(5):693–701.
[20] Du Y L,Zhao J,Jia X M,et al.Observation of RNA interference silencing IGF-1R expression on A549 cell apoptosis and chemotherapy sensitivity[J].Chinese Journal of Cancer Prevention and Treatment,2013,20(11):835–839.
[21] Gao H,Shi J,Ge S F,et al.Suppression of insulin-like growth factor-1 receptor by RNA interference inhibits cell growth in vitro and induces chemosensitization of HO8910PM cell to cisplatin[J].Chinese Journal of Obstetrics and Gynecology,2008,43(1):45–49.
[22] Geourjon C,Deléage G.SOPMA:Significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments[J].Computer Applications in the biosciences:CABIOS,1995,11(6):681–684.
[23] Schwede T,Kopp J,Guex N, et al.Swiss-model:An automated protein homology-modeling server[J].Nucleic acids research,2003,31(13):3881–3885.
[24] Ma M H.The SCD gene cloning and bioinformatics analysis in Smaindroina [D].Zhonshan:Zhejiang Ocean University,2013.
[25] Li N.Cloning and sequence analysis of cDNA encoding xyloglucan transglycoslasc for Anthocephalus chinensis [D].Beijing:Beijing Forestry University,2008.
[26] Ma J G.Principles of Genetic Engineering [M].Xian:Xian Jiaotong University Press,2013.32–33.
[27] Zhang J Z,Wan Y K.Progress on molecular mechanisms of chronological ageing in model organism Saccharomyces cerevisiae[J].Progress in Biochemistry and Biophysics,2011,38(2):119–126.

Bioinformatics Analysis of Bovine Insulin-like Growth Factor-1 Receptor

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