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Sequence Analysis of ALDH3B1 Gene in Human Pleural Mesothelioma
Lu Qiu, Li Zhang, Haiyan Yang, Na Yang, An Jiang, Qiong Yu, YingxuanWen, Yi Zhao, Shengjie Yang
2020, 35(6):
62-75.
The result was discovered that gene mutation of ALDH3B1 gene was associated with the development of human pleural mesothelioma through the DE novo sequencing of human pleural mesothelioma DNA. By PCR amplification, the ALDH3B1 gene fragment of 52 human pleural mesothelioma samples and 11 non-pleural mesothelioma samples were obtained, and the mutation of ALDH3B1 gene of human pleural mesothelioma was verified by next-generation sequencing technology. The results of a series of comparison of ALDH3B1 gene fragments showed that: ① Compared patients of non-pleural mesothelioma, patients of human pleural mesothelioma had more ALDH3B1 gene mutation sites, with 9 gene replacement sites, 1 gene inversion point and 3 gene fragments severely missing. ②Among the sites with a higher mutation rate, G-A replacement occurred at 67789454 bp, with a mutation rate of 12.96%. The G-A replacement occurred at the 67789380bp site with a mutation rate of 9.43%. The G-A replacement occurred at 67789589 bp, with a mutation rate of 7.69%. C-T replacement occurred at 67789407 bp, with a mutation rate of 7.55%. ③ The patients with more mutation sites were XL1, XL36, XL47 and XLZCH, each of which had four mutation sites. The patient Z17, with non-pleural mesothelioma, had three mutation sites and severe gene fragment deletion. ④ Without other mutation occurring, XL44ZL and XL44 were adjacent tissues and cancer tissues of the same case, and G-A replacement was detected at 67789454 bp. ⑤ No gene mutation occurred at 67789292 bp . The results showed that the occurrence and development of human pleural mesothelioma were related to the mutation of ALDH3B1 gene. Whether ALDH3B1 gene can be served a biomarker for human pleural mesothelioma still needs to be further verified by expanding the sample size.
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