Effects of Five Treatments on Detoxification of Tissue Culture Seedlings of Strawberry with Cream

Journal of Chuxiong Normal University ›› 2021, Vol. 36 ›› Issue (3): 80-85.

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Effects of Five Treatments on Detoxification of Tissue Culture Seedlings of Strawberry with Cream

LI Guoshu^1,2, XU Chengdong^1,2, WANG Zhengji¹, FAN Shuguo^1,2, GUAN Qingbing¹

1. School of Resources, Environment & Chemistry, Chuxiong Normal University, Chuxiong, Yunnan Province 675000;
2. Institute for Bio-resources Research and Development of Central Yunnan Plateau, Chuxiong, Yunnan Province 675000

Received:2020-05-03 Online:2021-05-20 Published:2021-07-23

Abstract

Abstract: To screen out the best method of detoxification culture and virus detection of tissue culture seedling of Strawberry of cream, the old leaves of strawberry with whipped cream are used as the explants to seed out of the tube seedlings and develop the tube seedlings respectively. Five methods – ribavirin in medium composition, day and night temperature treatment, high temperature, low temperature cultivation, seedling stem tip cultivation – are used to sterilize the tube seedlings, which are then treated with indicator plant daub mandala, amaranth, thousand-day red Chrysanthemum. Three weeks after transplanting, the external morphology was detection. The results showed that 1) the detoxification effect was the best after 40℃ high temperature treatment combined with stem tip culture; 2) the medium containing 25 mg/L triazole riboside was beneficial to the detoxification of strawberry butters; and 3) the plant Mandala is more sensitive to the strawberry virus than amaranth and thousand-day red Chrysanthemum and is thus the most convenient method for observation of external morphology after transplanting of detoxified seedlings.

Key words: strawberry of cream cv., aseptic tube seedling, detoxification technology, indicating plant

CLC Number:

S668.4

LI Guoshu, XU Chengdong, WANG Zhengji, FAN Shuguo, GUAN Qingbing. Effects of Five Treatments on Detoxification of Tissue Culture Seedlings of Strawberry with Cream[J]. Journal of Chuxiong Normal University, 2021, 36(3): 80-85.

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Effects of Five Treatments on Detoxification of Tissue Culture Seedlings of Strawberry with Cream

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